Pyocyanin isolated from a marine microbial population: synergistic production between two distinct bacterial species and mode of action.
نویسندگان
چکیده
Marine microbial populations collected from the Hawaiian Islands were screened for antimicrobial activity. A blue metabolite was identified from mixed cell cultures, but production was not evident in pure cultures. Experiments designed to probe the synergistic role of the microorganisms are presented. Full characterization of the blue natural product, pyocyanin, is provided including corrections made to 1H and 13C-NMR assignments of the molecule misreported in the chemical literature and yeast transcriptome analysis. The transcriptional effects were consistent with the compound's purported role as an inducer of oxidative stress and damage and illustrates the overall potential of the method to reveal the primary biological/cellular effects of a natural product. The experiments outlined here might serve as a general paradigm for identification of natural products arising from microbial communities and investigation of their respective interactions.
منابع مشابه
Synergistic bactericidal activity of a naturally isolated phage and ampicillin against urinary tract infecting Escherichia coli O157
Objective(s): Bacteriophages are infectious replicating entities that are under consideration as antimicrobial bioagents to control bacterial infections. As an alternative or supplement to antibiotics, bacteriophages can be used to circumvent the resistance to existing antibiotics. The aim of this study was to assess the synergistic effect of a naturally isolated phage...
متن کاملDetermination of bacterial species isolated from infected lesions of cutaneous Leishmaniasis
Background: Cutaneous leishmaniasis is a common parasitic disease in Isfahan province. A common complication of cutaneous leishmaniasis is bacterial superinfection, which may intensify the disease and produce scarring. Objective: The aim of this study was to determine the bacterial species isolated from infected lesions of cutaneous leishmaniasis. Patients and Methods: Two hundred out of ...
متن کاملExistance of Microbial Species in Vermicomposts Derived from Mixed Sesame Crust and Cow Manure Treatments
Introduction: The presence of pathogenic microbial agents and pathogens in organic fertilizers causes health problems and disease transmission.Therefore , the aim of this study was to identify bacterial and fungal species present in vermicompost production. Materials and Methods: This experimental study was conducted in pilot scale in the laboratory of Public Health School in Shahid Sadoughi U...
متن کاملEffect of fetal and adult bovine serum on pyocyanin production in Pseudomonas aeruginosa isolated from clinical and soil samples
Objective(s): Pyocyanin is a blue-greenish redox-active pigment, produced by Pseudomonas aeruginosa, with a wide range of biological and biotechnological applications. Pyocyanin biosynthesis is regulated by the quorum-sensing (QS) system in which the expression of QS genes and QS-controlled virulence genes may be affected by serum as a complex medium. In the current study, effects of adult bovi...
متن کاملApplications of Essential Oils in Food Preservation
Many food products are perishable by nature and require protection from spoilage during their preparation, storage and distribution to give them desired shelf-life. Because food products are now often sold in areas of the world far distant from their production sites, the need for extended safe shelf-life for these products has also expanded. Currently, there is a strong debate about the safety...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemistry & biology
دوره 13 12 شماره
صفحات -
تاریخ انتشار 2006